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This review was inspired by a January 2024 conference held at Friday Harbor Laboratories, WA, honoring the pioneering work of A.O. Dennis Willows, who initiated research on the sea slug Tritonia diomedea (now T. exsulans). A chance discovery while he was a student at a summer course there has, over the years, led to many insights into the roles of identified neurons in neural circuits and their influence on behavior. Among Dennis’s trainees was Peter Getting, whose later groundbreaking work on central pattern generators profoundly influenced the field and included one of the earliest uses of realistic modeling for understanding neural circuits. Research on Tritonia has led to key conceptual advances in polymorphic or multifunctional neural networks, intrinsic neuromodulation, and the evolution of neural circuits. It also has enhanced our understanding of geomagnetic sensing, learning and memory mechanisms, prepulse inhibition, and even drug-induced hallucinations. Although the community of researchers studying Tritonia has never been large, its contributions to neuroscience have been substantial, underscoring the importance of examining a diverse array of animal species rather than focusing on a small number of standard model organisms. 

Abstract Social predation is a common strategy used by predators to subdue and consume prey. Animals that use this strategy have many ways of finding each other, organizing behaviors and consuming prey. There is wide variation in the extent to which these behaviors are coordinated and the stability of individual roles. This study characterizes social predation by the nudibranch mollusc,Berghia stephanieae, which is a specialist predator that eats only the sea anemone,Exaiptasia diaphana. A combination of experimental and modeling approaches showed thatB. stephanieaedoes predate uponE. diaphanain groups. The extent of social feeding was not altered by length of food deprivation, suggesting that animals are not shifting strategies based on internal state. It was unclear what cues the individualBerghiaused to find each other; choice assays testing whether they followed slime trails, were attracted to injured anemones, or preferred conspecifics feeding did not reveal any cues. Individuals did not exhibit stable roles, such as leader or follower, rather the population exhibited fission-fusion dynamics with temporary roles during predation. Thus, theBerghiaprovides an example of a specialist predator of dangerous prey that loosely organizes social feeding, which persists across hunger states and uses temporary individual roles; however, the cues that it uses for aggregation are unknown. Significance StatementSocial predation is a strategy to hunt dangerous prey and minimize injury. Many nudibranchs specialize as predators of cnidarians, which are dangerous to them. Although nudibranchs are typically characterized as solitary hunters, we provide evidence that social predation strategies may be used by a species that specializes on one species of sea anemone. The study showed that the individual sea slugs assumed temporary roles for establishing groups and that the group dynamics were unstable. However, the cues that the nudibranchs use to aggregate remain elusive. 

Abstract Creating a high-resolution brain atlas in diverse species offers crucial insights into general principles underlying brain function and development. A volume electron microscopy approach to generate such neural maps has been gaining importance due to advances in imaging, data storage capabilities, and data analysis protocols. Sample preparation remains challenging and is a crucial step to accelerate the imaging and data processing pipeline. Here, we introduce several replicable methods for processing the brains of the gastropod mollusc,Berghia stephanieaefor volume electron microscopy. Although high-pressure freezing is the most reliable method, the depth of cryopreservation is a severe limitation for large tissue samples. We introduce a BROPA-based method using pyrogallol and methods to rapidly process samples that can save hours at the bench. This is the first report on sample preparation and imaging pipeline for volume electron microscopy in a gastropod mollusc, opening up the potential for connectomic analysis and comparisons with other phyla. 

Neuronal networks are the standard heuristic model today for describing brain activity associated with animal behavior. Recent studies have revealed an extensive role for a completely distinct layer of networked activities in the brain—the gene regulatory network (GRN)—that orchestrates expression levels of hundreds to thousands of genes in a behavior-related manner. We examine emerging insights into the relationships between these two types of networks and discuss their interplay in spatial as well as temporal dimensions, across multiple scales of organization. We discuss properties expected of behavior-related GRNs by drawing inspiration from the rich literature on GRNs related to animal development, comparing and contrasting these two broad classes of GRNs as they relate to their respective phenotypic manifestations. Developmental GRNs also represent a third layer of network biology, playing out over a third timescale, which is believed to play a crucial mediatory role between neuronal networks and behavioral GRNs. We end with a special emphasis on social behavior, discuss whether unique GRN organization andcis-regulatory architecture underlies this special class of behavior, and review literature that suggests an affirmative answer.